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Big News! This CHO Cell Feed is So Outstanding

Views: 0     Author: Site Editor     Publish Time: 2023-02-07      Origin: Site

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The EmCD CHO® 101 Feed (“101 Feed” for short) independently developed by Eminence is specially designed for Chinese hamster ovary (CHO) cells, and can significantly improve cell growth, enhance the yield and quality and reduce the production costs when used in combination with the EmCD CHO® 101 Basal Medium (“101 medium series” for short). The 101 Feed can be used with most commercially available CHO basal medium series besides 101 medium series, providing more flexible choices for users. It is a chemically defined medium without animal-derived components, which renders and ensures the safety and consistency of the monoclonal antibodies or recombinant proteins manufactured.


1. Splendid Performance

Compared to commercially available culture media, 101 medium series have incomparable advantages in terms of cell growth, yield and quality. With appropriate cell density, it is very possible to improve the yield through process optimization. In addition, consistent quality can eliminate customers' concerns for a change in medium.


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Figure 1 Viable Cell Density, Viability and Yield of Different CHO-ZN Cells Cultured in 101 Basal Medium + 101 Feed and M + H (M + H is a Typical Combination of Commercially Available Media for CHO-ZN Cells) under the Fed-Batch Culture Conditions


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Figure 2 Viable Cell Density, Viability and Yield of Different CHO-K1 Cells Cultured in 101 Basal Medium + 101 Feed and G + H (G + H is a Typical Combination of Commercially Available Media for CHO-K1 Cells) under the Fed-Batch Culture Conditions


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Figure 3 Product Quality with 101 Medium + 101 Feed and M + H


2. Excellent Universality

In virtue of its universality, the 101 medium series can be freely used for different CHO cells such as CHO-K1, CHOZN, CHO-DG44 and CHO-S. After process optimization, unexpected yield and quality performance can be attained.

They can also be used for different clones derived from the same host cell.


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Figure 4 Viable Cell Density, Viability and Yield of Different CHO Cells Cultured in 101 Basal Medium + 101 Feed under Fed-Batch Culture Conditions



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Figure 5 Growth and Expression of Different Sub-clones Derived from the Same Host Cell with 101 Basal Medium + 101 Feed


3. Sound stability

The 101 medium series has been manufactured for multiple batches. Fed-batch culture shows that the media from different batches contribute to basically consistent cell growth and yield, ensuring a stable supply of products.


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Figure 6 Viable Cell Density, Viability and Yield of CHO-ZN Cells Cultured in 101 Basal Medium + 101 Feed from Different Batches under Fed-Batch Culture Conditions


4. More sophisticated quality system:

Full animal origin-free lifecycle supply chain

Selected raw materials and contact materials with a “Animal-free Statement”

Animal origin-free control throughout the factory

Animal-free Statement provided with each batch of finished products


Quality commitment

Culture media are manufactured in compliance with the current GMP, ISO 9001 and ISO 13485.


Controlled manufacturing environment

Dry powder products are manufactured in a Class D clean environment with special controls on humidity, temperature, differential pressure and airborne particles to prevent contamination, cross-contamination and confusion.


Diversified manufacturing processes

Needle grinding manufacturing process

Hammer grinding manufacturing process


Inquiries are welcome!

Product

Article No.

Packing specification

Storage

Validity period

EmCD CHO® 101 Basal Medium

L10100.1000

1L

Store at 2-8℃, protected from light

12 months

P10100.0010

10L

Store at 2-8℃, protected from light

18 months

P10100.0100

100L

Store at 2-8℃, protected from light

18 months

P10100.0500

500L

Store at 2-8℃, protected from light

18 months

EmCD CHO® 101 Feed A Medium

P10112.0001

1L

Store at 2-8℃, protected from light

18 months

P10112.0010

10L

Store at 2-8℃, protected from light

18 months

P10112.0050

50L

Store at 2-8℃, protected from light

18 months

P10112.12kg

12kg

Store at 2-8℃, protected from light

18 months

EmCD CHO® 101 Feed B Medium

P10114. 0001

1L

Store at 2-8℃, protected from light

18 months

P10114. 0005

5L

Store at 2-8℃, protected from light

18 months

P10114. 0020

20L

Store at 2-8℃, protected from light

18 months

P10114.6kg

6kg

Store at 2-8℃, protected from light

18 months




Supported Compliance

Eminence

Company

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