EmCD CHO® 121 Basal Medium

EmCD CHO^® 121 Basal Medium is precisely formulated to tackle the challenges of scaling up CHO-S cells in bioreactors, ensuring the highest quality and expression. This medium has also been shown to significantly improve protein expression in CHO-K1 cells as well in fed-batch process. This chemically defined products, devoid of protein and animal-origin components, guarantee high productivity and consistent performance, and simplify transfer to large-scale manufacturing.

Key Features

• Achieve Higher Expressions: When paired with EmCD CHO^® 121 Feed, EmCD CHO^® 121 Basal Medium is fortified to help maximize CHO cell growth and protein expression in fed-batch culture, yielding up to 4.7 times higher product expression compared to just glucose-supplemented batch cultures by day 14.

• Streamline Scalability and Accelerate Process Development: EmCD CHO^® 121 Series Medium deliver the power to achieve a high cell density swiftly, outperforming commercial media in both shake flasks and bioreactors. The remarkable cell growth rates make it an ideal choice for scale-up. Alongside its compatibility with other CHO cell lines, including CHO-K1, CHO-S, and CHO-DG44, it provides a versatile and efficient way to develop processes.

Considering to Use EmCD CHO^® 121 Basal Medium

• When addressing rapid cell death, declining cell density, or low expression in the fed-batch process scale-up of CHO-S cell lines.

• Seeking a platform medium adaptable to various CHO cell subtypes for growth and expression.

Product Information

Animal-component free, Protein free, Growth-factors free, Glutamine free

Utilizing the EmCD CHO^® 121 Basal Medium in conjunction with the EmCD CHO^® 121 Feed for fed-batch cultures of CHO-S cell lines leads to a significant enhancement in cell conditions compared to batch cultures that solely add glucose. The highest live cell density reaches 25×10^6 cells/mL, with a viability rate exceeding 90% on Day 14, and a product expression that increases by 4.7 times

Utilizing the EmCD CHO^® 121 Basal Medium in conjunction with the EmCD CHO^® 121 Feed for fed-batch cultures of CHO-K1 cell lines has better growth curve to that of commercialized media

The cell viability maintained in fed-batch culture process with the EmCD CHO^® 121 series medium is superior to that of commercialized media, with the viability in Day 14 higher than 90%

The Titer of EmCD CHO^® 121 series culture medium in fed-batch culture process of CHO-K1 cells is 19% higher than the leading international media, reaching 6.9g/L

Storage Conditions: 2°C to 8°C, protect from light

Shipping Conditions: Ambient

Expiration Date of Liquid Product: 12 Months

Expiration Date of Powder Product: 24 Months