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| Catalog No. | Product Name | Appearance | Volume |
| P10710.0001 | EmCD CHO® 121 Feed A | Powder | 1L |
| P10710.0010 | EmCD CHO® 121 Feed A | Powder | 10L |
| P10710.0050 | EmCD CHO® 121 Feed A | Powder | 50L |
| P10710.12kg | EmCD CHO® 121 Feed A | Powder | 12kg |
| P10711.0001 | EmCD CHO® 121 Feed B | Powder | 1L |
| P10711.0005 | EmCD CHO® 121 Feed B | Powder | 5L |
| P10711.0020 | EmCD CHO® 121 Feed B | Powder | 20L |
| P10711.6kg | EmCD CHO® 121 Feed B | Powder | 6kg |
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Product Overview
EmCD CHO® 121 Feeds are precisely formulated to tackle the challenges of scaling up CHO-S cells in bioreactors, ensuring the highest quality and expression. These medium have also been shown to significantly improve protein expression in CHO-K1 cells as well in fed-batch process. These chemically defined products, devoid of protein and animal-origin components, guarantee high productivity and consistent performance, and simplify transfer to large-scale manufacturing.
Key Features
Achieve Higher Expressions: Utilizing the EmCD CHO® 121 Basal Medium in conjunction with the EmCD CHO® 121 Feed can help maximize CHO cell growth and protein expression in fed-batch culture, yielding up to 4.7 times higher product expression compared to just glucose-supplemented batch cultures by day 14.
Streamline Scalability and Accelerate Process Development: EmCD CHO® 121 Series Medium deliver the power to achieve a high cell density swiftly, outperforming commercial media in both shake flasks and bioreactors. The remarkable cell growth rates make it an ideal choice for scale-up. Alongside its compatibility with other CHO cell lines, including CHO-K1, CHO-S, and CHO-DG44, it provides a versatile and efficient way to develop processes.
Considering to Use EmCD CHO® 121 Feed
When addressing rapid cell death, declining cell density, or low expression in the fed-batch process scale-up of CHO-S cell lines.
Seeking a platform medium adaptable to various CHO cell subtypes for growth and expression.
Product Information
| Type: | Chemically Defined Medium |
| Cell Line: | CHO-S, CHO-K1, CHOZN, CHO-DG44 |
| Appearance: | Powder |
| Packaging: | Barrel |
| Specification: | Feed A: 1L, 10L, 50L, 12kg Feed B: 1L, 5L, 20L, 6kg |
Components
Animal-component free, Protein free, Growth-factors free, Glutamine free
Data Chart
The cell growth in shake flasks and reactors using the EmCD CHO® 121 series medium is comparable to that achieved with commercialized media, with the peak live cell density reaching 28.4×10^6 cells/mL 
The cell viability maintained in shake flasks and reactors with the EmCD CHO® 121 series medium is superior to that of commercialized media 
In comparison to leading international commercial media, the EmCD CHO® 121 series medium demonstrates consistent yields during the scale-up process of CHO-S cells from shake flasks to reactors. In contrast, the yield from commercial media in reactor processes decreases by 33% 
The key quality attribute SEC of products from the EmCD CHO® 121 series medium is also comparable to commercialized media, with a dominant peak reaching up to 97%, and minimal fragments and aggregates (less than 3%) 
The key quality attribute CEX of products from the EmCD CHO® 121 series medium is on par with commercialized media, showcasing a lower acidic peak (less than 20%) and a higher neutral peak (over 70%) 
The mannose content (Man5) in the EmCD CHO® 121 series medium in shake flasks and reactors is less than 2%, which is lower than that of leading international commercial media
Storage & Logistic
Storage Conditions: 2°C to 8°C, protect from light
Shipping Conditions: Ambient
Retest Date of Powder Product: 18 Months
