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EmCD CHO® 121 Feed

Catalog No.

Product Name

Appearance

Volume

P10710.0001

EmCD CHO® 121 Feed A

Powder

1L

P10710.0010

EmCD CHO® 121 Feed A

Powder

10L

P10710.0050

EmCD CHO® 121 Feed A

Powder

50L

P10710.12kg

EmCD CHO® 121 Feed A

Powder

12kg

P10711.0001

EmCD CHO® 121 Feed B

Powder

1L

P10711.0005

EmCD CHO® 121 Feed B

Powder

5L

P10711.0020

EmCD CHO® 121 Feed B

Powder

20L

P10711.6kg

EmCD CHO® 121 Feed B

Powder

6kg

Model:
Volume:
Availability:

Product Overview

EmCD CHO® 121 Feeds are precisely formulated to tackle the challenges of scaling up CHO-S cells in bioreactors, ensuring the highest quality and expression. These medium have also been shown to significantly improve protein expression in CHO-K1 cells as well in fed-batch process. These chemically defined products, devoid of protein and animal-origin components, guarantee high productivity and consistent performance, and simplify transfer to large-scale manufacturing.


Key Features

  • Achieve Higher Expressions: Utilizing the EmCD CHO® 121 Basal Medium in conjunction with the EmCD CHO® 121 Feed can help maximize CHO cell growth and protein expression in fed-batch culture, yielding up to 4.7 times higher product expression compared to just glucose-supplemented batch cultures by day 14.

  • Streamline Scalability and Accelerate Process Development: EmCD CHO® 121 Series Medium deliver the power to achieve a high cell density swiftly, outperforming commercial media in both shake flasks and bioreactors. The remarkable cell growth rates make it an ideal choice for scale-up. Alongside its compatibility with other CHO cell lines, including CHO-K1, CHO-S, and CHO-DG44, it provides a versatile and efficient way to develop processes.


Considering to Use EmCD CHO® 121 Feed 

  • When addressing rapid cell death, declining cell density, or low expression in the fed-batch process scale-up of CHO-S cell lines.

  • Seeking a platform medium adaptable to various CHO cell subtypes for growth and expression.


Product Information

Type:

Chemically Defined Medium

Cell Line:CHO-S, CHO-K1, CHOZN, CHO-DG44
Appearance:Powder
Packaging:Barrel
Specification:

Feed A: 1L, 10L, 50L, 12kg

Feed B: 1L, 5L, 20L, 6kg


Components

Animal-component free, Protein free, Growth-factors free, Glutamine free


Data Chart

图片7 The cell growth in shake flasks and reactors using the EmCD CHO® 121 series medium is comparable to that achieved with commercialized media, with the peak live cell density reaching 28.4×10^6 cells/mL
图片1 The cell viability maintained in shake flasks and reactors with the EmCD CHO® 121 series medium is superior to that of commercialized media
图片9 In comparison to leading international commercial media, the EmCD CHO® 121 series medium demonstrates consistent yields during the scale-up process of CHO-S cells from shake flasks to reactors. In contrast, the yield from commercial media in reactor processes decreases by 33%


图片10 The key quality attribute SEC of products from the EmCD CHO® 121 series medium is also comparable to commercialized media, with a dominant peak reaching up to 97%, and minimal fragments and aggregates (less than 3%)
图片11 The key quality attribute CEX of products from the EmCD CHO® 121 series medium is on par with commercialized media, showcasing a lower acidic peak (less than 20%) and a higher neutral peak (over 70%)
图片12

The mannose content (Man5) in the EmCD CHO® 121 series medium in shake flasks and reactors is less than 2%, which is lower than that of leading international commercial media


Storage & Logistic

Storage Conditions: 2°C to 8°C, protect from light

Shipping Conditions: Ambient

Retest Date of Powder Product: 18 Months


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  • Material Safety Data Sheet ( MSDS)
  • Product and Service Manual
  • Certificate of Analysis ( CoA/Animal Origin Statment )
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